WISP1 polyclonal antibody
产品名称： WISP1 polyclonal antibody
英文名称： WISP1 polyclonal antibody
产品编号： PAB4835
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of WISP1.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to internal region of human WISP1.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Regulation of the Wnt signaling pathway by disabled-2 (Dab2).
Hocevar BA, Mou F, Rennolds JL, Morris SM, Cooper JA, Howe PH.EMBO J. 2003 Jun 16;22(12):3084-94.
- 2.
- Human WISP1v, a member of the CCN family, is associated with invasive cholangiocarcinoma.
Tanaka S, Sugimachi K, Kameyama T, Maehara S, Shirabe K, Shimada M, Wands JR, Maehara Y.Hepatology. 2003 May;37(5):1122-9.
- 3.
- Overexpression of WISP-1 down-regulated motility and invasion of lung cancer cells through inhibition of Rac activation.
Soon LL, Yie TA, Shvarts A, Levine AJ, Su F, Tchou-Wong KM.J Biol Chem. 2003 Mar 28;278(13):11465-70. Epub 2003 Jan 15.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis of HL-60 cell lysate (35 ug/lane) with WISP1 polyclonal antibody (Cat # PAB4835).
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with WISP1 polyclonal antibody (Cat # PAB4835) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Entrez GeneID:
- 8840
- Protein Accession#:
- WISP1_HUMAN
- Gene Name:
- WISP1
- Gene Alias:
- CCN4,WISP1c,WISP1i,WISP1tc
- Gene Description:
- WNT1 inducible signaling pathway protein 1
- Omim ID:
- 603398
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue, and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase. It is 83% identical to the mouse protein at the amino acid level. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq
- Other Designations:
- WNT1 induced secreted protein 1,Wnt-1 inducible signaling pathway protein 1,wnt-1 signaling pathway protein 1