ANAPC1 (phospho S355) polyclonal antibody
产品名称： ANAPC1 (phospho S355) polyclonal antibody
英文名称： ANAPC1 (phospho S355) polyclonal antibody
产品编号： PAB10017
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of ANAPC1.
- Immunogen:
- Synthetic phosphopeptide corresponding to residues surrounding S355 of human ANAPC1.
- Host:
- Rabbit
- Reactivity:
- Bovine, Chicken, Chimpanzee, Dog, Human, Mouse, Rat
- Specificity:
- This antibody is specific to phosphorylated human APC1 protein at the pS355 residue. Minimal reactivity is expected with the non-phosphorylated form of the protein.
- Form:
- Liquid
- Storage Buffer:
- In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- ELISA (1:10000-1:40000)
Western Blot (1:200-1:2000)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Mitotic regulation of the human anaphase-promoting complex by phosphorylation.
Kraft C, Herzog F, Gieffers C, Mechtler K, Hagting A, Pines J, Peters JM.EMBO J. 2003 Dec 15;22(24):6598-609.
- 2.
- Characterisation of the human APC1, the largest subunit of the anaphase-promoting complex.
Jorgensen PM, Graslund S, Betz R, Stahl S, Larsson C, Hoog C.Gene. 2001 Jan 10;262(1-2):51-9.
- Applications
- Western Blot (Cell lysate)
- Western blot using ANAPC1 (phospho S355) polyclonal antibody (Cat # PAB10017) shows detection of a band ~215 KDa corresponding to phosphorylated human ANAPC1 (arrowhead).
Lane 1 shows lysate from asynchronous cells.
Lane 2 shows lysate from cells treated with thymidine to synchronize cells at the G1/S boundary.
Lane 3 shows lysate from cells treated with nocodazole to synchronizecells at the M phase.
Phosphorylated ANAPC1 is mostly present only in cell preparations arrested at cell division.
Each lane contains approximately 30 ug of HeLa S3 whole cell lysates separated by 12.5% SDS-PAGE followed by transfer to nitrocellulose.
After blocking with 5% non-fat drymilk in TTBS, the membrane was probed with the primary antibody diluted to 1 : 500 for 1 h at room temperature followed by washes and reaction with a 1 : 5,000 dilution of HRP Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.
ECL reagent was used for detection.
Data contributed by Bing Li, UT South w
- Entrez GeneID:
- 64682
- Protein Accession#:
- Q9H1A4;NP_073153
- Gene Name:
- ANAPC1
- Gene Alias:
- APC1,MCPR,TSG24
- Gene Description:
- anaphase promoting complex subunit 1
- Omim ID:
- 608473
- Gene Ontology:
- Hyperlink
- Gene Summary:
- ANAPC1 is 1 of at least 10 subunits of the anaphase-promoting complex (APC), which functions at the metaphase-to-anaphase transition of the cell cycle and is regulated by spindle checkpoint proteins. The APC is an E3 ubiquitin ligase that targets cell cycle regulatory proteins for degradation by the proteasome, thereby allowing progression through the cell cycle.[supplied by OMIM
- Other Designations:
- anaphase-promoting complex 1 (meiotic checkpoint regulator)
- Gene Pathway