ABL1 (phospho Y245) polyclonal antibody
产品名称： ABL1 (phospho Y245) polyclonal antibody
英文名称： ABL1 (phospho Y245) polyclonal antibody
产品编号： PAB7918
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
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- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of ABL1.
- Immunogen:
- Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y245 of human ABL1.
- Host:
- Rabbit
- Reactivity:
- Human, Mouse, Rat
- Specificity:
- This peptide sequence has high homology to the conserved site in rat and mouse c-Abl, as well as in viral Abl and BCR-Abl fusion protein.
- Form:
- Liquid
- Storage Buffer:
- In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
- Storage Instruction:
- Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- ELISA (1:2000)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Autoinhibition of c-Abl.
Pluk H, Dorey K, Superti-Furga G.Cell. 2002 Jan 25;108(2):247-59.
- 2.
- c-Abl has high intrinsic tyrosine kinase activity that is stimulated by mutation of the Src homology 3 domain and by autophosphorylation at two distinct regulatory tyrosines.
Brasher BB, Van Etten RA.J Biol Chem. 2000 Nov 10;275(45):35631-7.
- 3.
- Cycling, stressed-out and nervous: cellular functions of c-Abl.
Van Etten RA.Trends Cell Biol. 1999 May;9(5):179-86.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis of K-562 cells treated with pervanadate (1 mM) for 30 minutes (lanes 1 & 3). Some lanes were treated with alkaline phosphatase to remove phosphorylation on ABL1 (lanes 2 & 4), then the blots were probed with anti ABL1 (lanes 1 & 2), ABL1 (phospho Y245) polyclonal antibody (Cat # PAB7918) (lanes 3 & 4).
- Entrez GeneID:
- 25
- Gene Name:
- ABL1
- Gene Alias:
- ABL,JTK7,bcr/abl,c-ABL,p150,v-abl
- Gene Description:
- c-abl oncogene 1, receptor tyrosine kinase
- Omim ID:
- 189980
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The ABL1 protooncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Activity of c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. The t(9;22) translocation results in the head-to-tail fusion of the BCR (MIM:151410) and ABL1 genes present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function for ABL1. The ABL1 gene is expressed as either a 6- or 7-kb mRNA transcript, with alternatively spliced first exons spliced to the common exons 2-11. [provided by RefSeq
- Other Designations:
- Abelson murine leukemia viral (v-abl) oncogene homolog 1,OTTHUMP00000022375,OTTHUMP00000022376,bcr/c-abl oncogene protein,proto-oncogene tyrosine-protein kinase ABL1,v-abl Abelson murine leukemia viral oncogene homolog 1
- Related Disease
- Adenocarcinoma
- Alzheimer Disease
- Alzheimer disease
- Breast cancer
- Breast Neoplasms
- Cardiovascular Diseases
- Chronic Disease
- Diabetes Complications
- Esophageal Neoplasms
- Genetic Predisposition to Disease
- HIV Infections
- Leukemia, chronic myeloid
- Leukemia, Myelogenous, Chronic, BCR-ABL Positive
- Leukemia, Myeloid
- Leukemia, Myeloid, Chronic
- Metabolic Syndrome X
- Neoplasms
- Osteoporosis
- Ovarian cancer